Purification of a 29-kDa hemocyte proteinase of Sarcophaga peregrina
نویسندگان
چکیده
منابع مشابه
Purification and Characterization of an Inhibitor of the Cysteine Protease from the Hemolymph of Sarcophaga peregrina
The hemolymph of Sarcophaga peregrina (flesh fly) larvae was found to contain multiple inhibitors of hemocyte cysteine protease. One of them, named sarcocystatin A, was purified and found to be a mixture of the components sarcocystatin A, and AP in a molar ratio of 2:l. These components can exist in either the associated or dissociated form. The apparent heterogeneity of the protease inhibitors...
متن کاملPurification of lectin induced in the hemolymph of Sarcophaga peregrina larvae on injury.
A lectin was purified from the hemolymph of Sarcophaga peregrina larvae, obtained after injury of their body wall. This lectin agglutinated sheep red blood cells markedly and the hemagglutinating activity was inhibited by galactose and lactose. The active lectin was found to have a molecular weight of 190,000 and to consist of four alpha subunits and two beta subunits, with molecular weights of...
متن کاملAnalysis of the DNase I-hypersensitive site of a developmentally regulated 25-kDa protein gene of Sarcophaga peregrina.
Change in chromatin structure of a developmentally regulated gene of Sarcophaga peregrina (flesh fly) during development was investigated. This gene (25-kDa protein gene) was specifically activated in the fat body, but not the hemocytes of larvae in the middle of the third instar. The mRNA level in the fat body decreased thereafter, reaching one-fifth of the maximum level in the late third inst...
متن کاملA novel protease in the pupal yellow body of Sarcophaga peregrina (flesh fly). Its purification and cDNA cloning.
We purified a novel serine protease with a molecular mass of 26 kDa from Sarcophaga pupae. This protease appeared almost exclusively in the yellow body, an organ that develops temporarily in the pupae of dipteran insects and expands to form the adult midgut by engulfing the larval midgut. cDNA analysis revealed that this protease consists of 239 amino acid residues and has significant structura...
متن کاملMolecules participating in insect immunity of Sarcophaga peregrina
Pricking the body wall of Sarcophaga peregrina (flesh fly) larvae with a needle activated the immune system of this insect and induced various immune molecules, including antibacterial proteins, in the hemolymph. In this review, I summarize and discuss the functions of these immune molecules, with particular emphasis on the dual roles of some of these molecules in defense and development.
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1992
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1992.tb16711.x